کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2060107 1076299 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Saccharomyces cerevisiae K2 toxin requires acidic environment for unidirectional folding into active state
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Saccharomyces cerevisiae K2 toxin requires acidic environment for unidirectional folding into active state
چکیده انگلیسی


• Functionality of Saccharomyces cerevisiae K2 toxin is determined by the environmental temperature and pH.
• K2 protein is secreted in high level at conditions incompetent for display of toxicity.
• K2 toxin, produced at basic pH, can be reactivated by adjusting pH to acidic value.
• Functional toxin does not survive basic pH shift.

The secretion of toxic proteinaceous compounds is common phenomenon among yeast genera. Toxin properties are determined by natural habitats and depend on the extracellular conditions. We analyzed in details the effect of temperature and pH on the activity and stability of Saccharomyces cerevisiae K2 toxin. By performing large-scale and long-term analysis, pH value has been identified to be an essential factor for the acquisition of killer protein functionality. We have found that K2 killer toxin is produced in comparable levels at broad range of pH values, in clear contrast to activity profile of the protein. Protein, produced at basic pH conditions—incompatible with killer activity—can be recovered into active form by adjustment of pH to acidic values. Fully active protein is nevertheless sensitive toward pH shift: once established, activity is un-recoverably lost upon adjustment of pH to basic values. Acidic environment as an essential condition of maturation of active form of K2 killer protein is postulated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mycoscience - Volume 57, Issue 1, January 2016, Pages 51–57
نویسندگان
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