Application of one-step reverse transcription loop mediated isothermal amplification (reverse transcription LAMP) for rapid detection of fungal gene expression in pure culture mycelia and in planta

• RT-LAMP was applied to analysis of gene expression directly in pure culture mycelia.
• RT-LAMP was applied to analysis of fungal gene expression in infected plants.
• The novel method had a 60 fold higher sensitivity as RT-LAMP.
• No in vitro preparation of cDNA was necessary prior to the analysis.
• Usefulness of RT-LAMP was demonstrated in mycelia and infected plants.

A novel application for reverse transcription loop-mediated isothermal amplification (reverse transcription LAMP) was developed which enables rapid, accurate and inexpensive detection of gene expression in pure culture mycelia with no previous mRNA isolation necessary as well as in infected plant tissue after mRNA isolation. Usefulness of the method was demonstrated by detection of mRNA templates of the Fusarium graminearum housekeeping genes coding for Ef1α and β-tubulin, in mycelia and in planta.