کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2062288 1076588 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functional Characterization of the 5′-upstream Region of MTT5 Metallothionein Gene from Tetrahymena thermophila
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Functional Characterization of the 5′-upstream Region of MTT5 Metallothionein Gene from Tetrahymena thermophila
چکیده انگلیسی

Metallothioneins are ubiquitous small, cysteine-rich, metal-binding proteins that play important roles in intracellular metal homeostasis and detoxification. Very few data are available on the promoter region and the mechanism of metallothionein transcription in Protozoa. In this study, we focused on Tetrahymena thermophila MTT5 5’-flanking region. To define the sequence elements underlying the metal-responsiveness of this promoter, we constructed a series of deletions and mutations starting with a 1777 bp fragment immediately upstream of the start codon of MTT5. As a reporter gene we used the previously tested IAG52B surface antigen from the protozoan fish parasite Ichthyophthirius multifiliis. The results suggest that a region spanning between –300 bp and –274 bp, dubbed Tetrahymena thermophila Cadmium-Response-Element (TtCdRE), is necessary to elicit high-level expression of the transgene following induction with cadmium. This is the first demonstration by in vivo analyses of a regulatory element essential for Cd-mediated control of protozoan metallothionein gene expression, where the sequence GATA appears to be involved.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protist - Volume 161, Issue 1, January 2010, Pages 71–77
نویسندگان
, , , , , ,