کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2063139 | 1076652 | 2012 | 6 صفحه PDF | دانلود رایگان |
Genotypic and phenotypic analyses were performed on five Gram-negative, catalase and oxidase-positive, rod-shaped bacteria isolated from the gill and liver of four rainbow trout. Studies based on comparative 16S rRNA gene sequence analysis showed that the five new isolates shared 99.8–100% sequence similarity and that they belong to the genus Chryseobacterium. The nearest phylogenetic neighbours of the strain 701B-08T were Chryseobacterium ureilyticum F-Fue-04IIIaaaaT (99.1% 16S rRNA gene sequence similarity) and Chryseobacterium joosteii LMG 18212T (98.6%). DNA–DNA hybridization values between the five isolates were 91–99% and ranged from 2 to 53% between strain 701B-08T and the type strains of phylogenetically closely related species of Chryseobacterium. Strain 701B-08T had a DNA G + C content of 36.3 mol%, the major fatty acids were iso-C15:0, iso-C17:1ω9c, C16:1ω6c and iso-C17:0 3-OH and the predominant respiratory quinone was MK-6. The novel isolates were distinguished from related Chryseobacterium species by physiological and biochemical tests. The genotypic and phenotypic properties of the isolates from rainbow trout suggest their classification as representatives of a novel species of the genus Chryseobacterium, for which the name Chryseobacterium oncorhynchi sp. nov. is proposed. The type strain is 701B-08T (=CECT 7794T = CCUG 60105T).
Journal: Systematic and Applied Microbiology - Volume 35, Issue 1, February 2012, Pages 24–29