کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2065574 1076928 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A method for acetonitrile-free microcystin analysis and purification by high-performance liquid chromatography, using methanol as mobile phase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
A method for acetonitrile-free microcystin analysis and purification by high-performance liquid chromatography, using methanol as mobile phase
چکیده انگلیسی

Cyanobacteria produce a wide range of potent toxins, including hepatotoxic microcystins. HPLC methods for microcystin analysis and purification almost invariably include acetonitrile in the elution gradient mobile phase. The recent, acute, global acetonitrile shortage requires that adequate methods are available for microcystin analysis and purification without the need for acetonitrile. Here we present a convenient methanol-based method for effective HPLC analysis and purification of the toxins, with full separation of a range of microcystin variants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 54, Issue 6, November 2009, Pages 887–890
نویسندگان
, , , ,