کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067189 | 1077886 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of 60Â Hz electromagnetic fields on the activity of hsp70 promoter: An in vitro study
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
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چکیده انگلیسی
We have evaluated the effect of 60 Hz sinusoidal magnetic fields (MF) at 8 and 8 μT on expression of the luciferase gene contained in a gene construct labelled as Electromagnetic Field-plasmid (pEMF). The vector included the hsp70 promotor containing the 3 nCTCTn sequences previously described for the induction of hsp70 expression by magnetic fields, as well as the reporter of the luciferase gene. We also replicated the study of Lin et al. [Lin H, Blank M, Rossol-Haseroth K, Goodman R. Regulating genes with electromagnetic response elements. J Cell Biochem 2001;81(1):143-48]. The pEMF plasmid was transfected into HeLa and BMK16 cell lines that were later exposed to either MF or thermal shock (TS). An increased luciferase expression was found in both the cells exposed to MF and TS compared with their control groups (P < 0.05). Furthermore, the combined effect of MF and TS was also analyzed. A synergistic effect between two factors was observed for this co-exposure condition in terms of luciferase gene expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 33, Issue 3, March 2009, Pages 419-423
Journal: Cell Biology International - Volume 33, Issue 3, March 2009, Pages 419-423
نویسندگان
Abraham O. RodrÃguez de la Fuente, Juan M. Alcocer-González, J. Antonio Heredia-Rojas, IsaÃas Balderas-Candanosa, Laura E. RodrÃguez-Flores, Cristina RodrÃguez-Padilla, Reyes S. Taméz-Guerra,