کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067810 | 1077911 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cytoplasmic pH-dependent spreading of polymorphonuclear leukocytes: Regulation by pH of PKC subcellular distribution and F-actin assembly
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cytoplasmic pH-dependent spreading of polymorphonuclear leukocytes: Regulation by pH of PKC subcellular distribution and F-actin assembly Cytoplasmic pH-dependent spreading of polymorphonuclear leukocytes: Regulation by pH of PKC subcellular distribution and F-actin assembly](/preview/png/2067810.png)
چکیده انگلیسی
Cytoplasmic pH (pHi) plays an important role in the regulation of polymorphonuclear leukocyte (PMN) spreading, but the molecular mechanisms involved have long been obscure. In the present study, we investigated the pH-dependence of phorbol myristate acetate (PMA)-induced PMN spreading. A change in pHi alone did not induce spreading, but cytoplasmic alkalinization promoted the spreading induced by PMA, whereas acidification inhibited it. To further investigate the mechanism by which pHi affects cell spreading, we employed subcellular fractionation and immunoblot analyses to evaluate the effect of pH on the subcellular distribution of protein kinase C (PKC) and assembly of the actin cytoskeleton. We found that cytoplasmic alkalinization enhanced PKC membrane distribution and quantitatively up-regulated the actin cytoskeleton. On the other hand, cytoplasmic acidification was found to have effects on these signaling molecules that were opposite to those of cytoplasmic alkalinization. These results may provide a potential explanation for the pH-regulation of the PMA-induced PMN spreading.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 31, Issue 3, March 2007, Pages 279-288
Journal: Cell Biology International - Volume 31, Issue 3, March 2007, Pages 279-288
نویسندگان
Kingo Suzuki, Hideo Namiki,