کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2067957 1077920 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of mouse Sim2 gene on the cell cycle of PC12 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوفیزیک
پیش نمایش صفحه اول مقاله
Effect of mouse Sim2 gene on the cell cycle of PC12 cells
چکیده انگلیسی
Sim2 gene plays an important role in the pathogenesis of Down syndrome (DS). To observe the effect of mouse Sim2 (mSim2) on the cell cycle of PC12 cells in vitro and explore the role of Sim2 in the pathogenesis of DS, we cloned the full open reading frame of mSim2 into the pcDNA3 vector and transfected it into PC12 cells, before analysing the effect of mSim2 on the cell cycle. A eukaryotic expression vector of mSim2 (pcDNA3-mSim2) was successfully constructed. There was notable expression of mSim2 mRNA in the cells transfected with pcDNA3-Sim2. Flow cytometry showed that there were more cells in G0/G1 phase in the Sim2-transfected cells than that in the controls (P < 0.01), and significantly fewer in G2/M phase (P < 0.01). The mRNA and protein expressions of cyclin E decreased in the Sim2-transfected cells, while p27 expression increased significantly (P < 0.01). It is concluded that Sim2 may play an important role in the pathogenesis of DS by inhibiting the cell cycle, which is related to the decreased expression of cyclin E and increased expression of p27.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 30, Issue 4, April 2006, Pages 349-353
نویسندگان
, , , , ,