کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2068791 | 1078353 | 2011 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Functional imaging of membrane potential at the single mitochondrion level: Possible application for diagnosis of human diseases Functional imaging of membrane potential at the single mitochondrion level: Possible application for diagnosis of human diseases](/preview/png/2068791.png)
Functional biochemical tests are the gold standard for the diagnosis of mitochondria-related diseases. However, the availability of the biological samples from patients' tissues represents a severe limitation to the number of screenable enzymatic activities. In this study we developed a fluorescent probe-assisted microscopy protocol enabling to assess the ΔΨm-generating capacity by mitochondria immobilized on a glass surface at the single organelle resolution-level. The advantage of this assay over others is to scale-down the amount of the biological sample required to test in a short time the functional activity of all the components of the oxidative phosphorylation system without loss of accuracy. Furthermore, the distribution of a given enzymatic activity can also be evaluated within the mitochondrial population enabling to measure the level of functional heterogeneity of the respiratory chain dysfunction.
Journal: Mitochondrion - Volume 11, Issue 5, September 2011, Pages 764–773