کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2072558 1544713 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sex determination of ovine embryos by SRY and amelogenin (AMEL) genes using maternal circulating cell free DNA
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Sex determination of ovine embryos by SRY and amelogenin (AMEL) genes using maternal circulating cell free DNA
چکیده انگلیسی


• This is the first report of ovine fetal sex determination using both SRY and AMEL genes in maternal circulating cell free DNA in Ghezel breed.
• Application of both SRY and AMEL genes in maternal circulation may be useful, however, the main drawback of this method is the failure to detect the gender of twins.
• Using AMEL gene may overcome the contamination issue as human AMEL gene primer produces different product size from animal AMEL gene.
• Quantitation of a PCR product from a plasma sample of pregnant ewe against a verified DNA sample from the same dam in the quantitative real-time PCR using AMEL gene primers might be useful.

Simple and precise methods for sex determination in animals are a pre-requisite for a number of applications in animal production and forensics. Some of the existing methods depend only on the detection of Y-chromosome specific sequences. However, the detection of Y and X-chromosome specific sequences is advantageous. In the present study the accuracy of sex determination by SRY (sex-determining region Y) and AMEL (Amelogenin) gene detection was assessed using a polymerase chain reaction (PCR) of DNA extracted from free fetal cells in maternal blood, which is noninvasive for fetus and easier to collect. The PCR amplification of SRY primers produced a single band of 171 bp from ewes bearing a male fetus, whereas no band was amplified from the DNA extracted from ewes pregnant to a female fetus. Moreover, two bands of 182 and 242 bp in male and a single band of 242 in female fetuses were produced by AMEL gene primers in the PCR reaction. Using this technique 100% of samples were successfully sexed, excluding twins. In conclusion, we demonstrated that sex determination using DNA of free fetal cells in maternal plasma is efficient using both SRY and AMEL gene sequences. It also is evident that this method is not suitable for sex determination of twin pregnancies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 164, January 2016, Pages 9–13
نویسندگان
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