کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2072958 | 1544747 | 2013 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A proteomic approach to identify seminal plasma proteins in roosters (Gallus gallus domesticus) A proteomic approach to identify seminal plasma proteins in roosters (Gallus gallus domesticus)](/preview/png/2072958.png)
Considering the interest in avian semen processing and storage, the objective of this study was to identify the domestic fowl seminal plasma proteins using two-dimensional gel electrophoresis (2-DE) and mass spectrometry MS/MS. For three times in a 4-month period, seminal plasma was obtained from semen collected from four local male chickens (Gallus gallus domesticus) and prepared for two-dimensional polyacrylamide gel electrophoresis. A total of 83 spots were detected across all gels and analyzed by MALDI-TOF/TOF. Among these spots, 17 have been successfully identified. The most intensely stained spots were recognized as serum albumin, ovotransferrin, alpha-enolase, fatty acid binding protein, thioredoxin, trypsin inhibitor CITI-1 and gallinacin-9. From these proteins, two are characteristic of avian seminal plasma, the ovotransferrin and gallinacin-9, and one is specific of the Gallus species, the chicken trypsin inhibitor CITI-1.
Journal: Animal Reproduction Science - Volume 140, Issues 3–4, August 2013, Pages 216–223