کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2073253 1544755 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The association between testicular ghrelin receptor mRNA and serum testosterone levels in immunocastrated boars
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
The association between testicular ghrelin receptor mRNA and serum testosterone levels in immunocastrated boars
چکیده انگلیسی

Ghrelin, the endogenous ligand for the GH secretagogue receptor (GHS-R), has various functions. The expression of ghrelin and growth hormone secretagogue receptor type 1a (GHS-R1a) has been demonstrated in rat and human testis, and ghrelin also affects testosterone (T) secretion in vitro, suggesting a role for this molecule in the direct control of testis function. However, whether this signaling system is present in pig testis, and the association with serum T remains largely unexplored. In this study we investigated the relationship between serum T levels and ghrelin and GHS-R1a gene expression in the testes of intact and immunocastrated boars. Testicular tissue and serum samples were collected from seven intact boars and seven recombinant GnRH-I immunocastrated boars. GHS-R1a gene expression in the testis was higher in immunocastrated than intact boars (2.22 ± 0.71 vs. 0.53 ± 0.23; P < 0.01). Mean serum T levels were markedly lower in immunocastrated than intact boars (0.40 ± 0.04 vs. 2.10 ± 0.94 ng/mL, P < 0.01). Ghrelin gene expression in testis did not differ between both groups of boars. GHS-R1a gene expression was positively correlated with ghrelin gene expression (r = 0.68, P < 0.001), but negatively correlated with serum T concentrations (r = −0.83, P < 0.001). Overall, the existence of ghrelin and GHS-R1a gene expression in pig testis, and the increase in GHS-R1a gene expression in the testes and the inverse correlation with serum T in immunocastrated boars are highly suggestive of a role for ghrelin in the regulation of mammalian testicular function.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 135, Issues 1–4, November 2012, Pages 62–67
نویسندگان
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