کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2073810 | 1544793 | 2009 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Normal reproductive capacity of heifers that originated from in vitro fertilized embryos cultured with an antiviral compound
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Bovine viral diarrhea virus (BVDV) can associate with in vitro fertilized (IVF) bovine embryos despite washing and trypsin treatment. An antiviral compound, DB606 (2-(4-[2-imidazolinyl]phenyl)-5-(4-methoxyphenyl)furan), inhibits the replication of BVDV in bovine uterine tubal epithelial cells, Madin Darby bovine kidney cells, and fetal fibroblast cells. As well, DB606 in in vitro culture medium does not affect embryonic development. Antiviral-treated-IVF embryos placed into recipients developed into clinically normal calves. The objective of this project was to determine if these resultant heifer calves were capable of reproducing. Seven heifers from each of the treatment groups (natural breeding, IVF embryo, and IVF embryo cultured in DB606) of the previous study were used. At 20-27Â months of age, the heifers were exposed to a fertile bull in a single pasture during a 63Â d breeding season. Five of the seven heifers originating from natural breeding were pregnant 35Â d after removal of the bull and calved. All of the heifers resulting from transfer of untreated IVF embryos were pregnant at 35Â d; however, one aborted the fetus at 5-7Â months of gestation. All of the heifers derived from transfer of IVF embryos cultured in DB606 were pregnant and calved. Offspring from dams of all treatment groups were clinically normal at birth. Adjusted 205Â d weaning weights were not significantly different among the offspring of the treated and untreated dams. These results indicate that culture of bovine-IVF embryos in DB606 does not impair future reproductive capacity of resulting heifers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 113, Issues 1â4, July 2009, Pages 283-286
Journal: Animal Reproduction Science - Volume 113, Issues 1â4, July 2009, Pages 283-286
نویسندگان
M. Daniel Givens, Mylissa S.D. Marley, Kay P. Riddell, Patricia K. Galik, David A. Stringfellow,