کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2075294 1079328 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cephalosporin C acylase from Pseudomonas species: Production and enhancement of its activity by optimization of process parameters
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Cephalosporin C acylase from Pseudomonas species: Production and enhancement of its activity by optimization of process parameters
چکیده انگلیسی
The bacterium, Pseudomonas species was isolated from rhizosphere soil and screened out for its ability to produce cephalosporin C acylase (CCA). Cephalosporin C acylase has the potential to transform cephalosporin C to 7- aminocephalosporanic acid which is an important intermediate for production of broad-spectrum semi-synthetic cephalosporins. This article describes the selection of medium components and parameters like temperature, pH, carbon sources, nitrogen sources and inoculum level for the optimal production of cephalosporin C acylase by Pseudomonas species. The biotransformation was conducted utilizing all optimized parameters for the production of cephalosporin C acylase at the end of study. The amount of product 7- aminocephalosporanic acid formed was measured by glutaryl p-nitroanilide (GpNA), p-Dimethyl aminobenzadehyde (p-DAB) and HPLC assays. The CCA activity in Pseudomonas species was measured 33.1 and 35.5 U/mL using substrate Cephalosporin C (CPC) and glutaryl p-nitroanilide (GpNA) respectively at optimized parameters. The maximum CCA acylase activity was detected at 72 h of incubation. The highest CCA activity for Pseudomonas species 42.9 U/mL was detected at 72 h of incubation by HPLC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 4, Issue 4, October 2015, Pages 465-470
نویسندگان
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