کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2075425 | 1079333 | 2014 | 4 صفحه PDF | دانلود رایگان |
• Immobilization of glucose isomerase on smooth and rough glass beads.
• Immobilization yield is significant.
• Comparison of immobilization of glucose isomerase on smooth and rough glass beads.
Immobilization of glucose isomerase (GI) (EC 5.3.1.5) has been carried out on non-porous glass surfaces using γ-aminopropyltriethoxysilane and polyethyleneimine as an activator and gluteraldehyde as cross linking agent for its potential use. Polyethyleneimine was found to be a superior immobilization activator than γ-aminopropyltriethoxysilane. Further, the effects of rough and smooth surfaces of non-porous glass beads on immobilization of glucose isomerase have been investigated. Polyethyleneimine on rough glass beads was found to be better activator material than that of γ-aminopropyltriethoxysilane. The prime objective of study was to immobilize glucose isomerase on an improved surface of non-porous glass beads which will give a significantly higher immobilization yield. After optimizing the parameters the immobilization yield increased from an initial value 29.43% to 79.6%.
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Journal: Biocatalysis and Agricultural Biotechnology - Volume 3, Issue 3, July 2014, Pages 86–89