کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2075439 | 1079334 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preparation and characterization of cross-linked enzyme aggregates (CLEAs) of Brassica rapa peroxidase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Preparation and characterization of cross-linked enzyme aggregates (CLEAs) of Brassica rapa peroxidase Preparation and characterization of cross-linked enzyme aggregates (CLEAs) of Brassica rapa peroxidase](/preview/png/2075439.png)
چکیده انگلیسی
Peroxidase from white turnip (Brassica rapa) was immobilized as cross-linked enzyme aggregate (CLEA) via solvent precipitation and glutaraldehyde cross-linking. The nature of the precipitation solvent, enzyme and glutaraldehyde concentrations affected the formation and the activity of the enzyme aggregate. The aggregates resulting from cross-linking with glutaraldehyde produced insoluble catalytically active cross-linked enzyme aggregates. Acetone was selected as the more efficient solvent, giving the highest activity recovery (28%) and aggregation yield (78%). Immobilization was conducted for 3 h under agitation at 4 °C. Optimal conditions were determined as 2% (v/v) glutaraldehyde and 9:1 (v/v) enzyme to glutaraldehyde ratio. The highest activity recovery and aggregation yield were 42.5% and 82% respectively. CLEAs with enhanced thermal and acidic condition stabilities were formed when compared to the free enzyme. The cross-linked enzyme aggregates exhibited optimal pH and temperature in the range 6-7 °C and 40 °C, respectively. CLEAs of Brassica rapa peroxidase exhibited good stability in nonpolar organic solvents and they could be stored in pH 7 buffer with no activity loss at 4 °C for more than three months.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 4, Issue 2, April 2015, Pages 208-213
Journal: Biocatalysis and Agricultural Biotechnology - Volume 4, Issue 2, April 2015, Pages 208-213
نویسندگان
Nassima Tandjaoui, Amina Tassist, Mahmoud Abouseoud, Annabelle Couvert, Abdeltif Amrane,