کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2075560 | 1079341 | 2013 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Production, characterization and applications of organic solvent tolerant lipase by Pseudomonas aeruginosa AAU2 Production, characterization and applications of organic solvent tolerant lipase by Pseudomonas aeruginosa AAU2](/preview/png/2075560.png)
• Pseudomonas aerugniosa AAU2 produced extracellular lipase on Jatropha seed cake.
• AAU2 lipase exhibited remarkable solvent and detergent tolerance.
• The application of AAU2 lipase in synthesis of biodiesel was demonstrated.
• AAU2 lipase efficiently hydrolyzed phorbol ester.
Jatropha seedcake (JSC) represents an important environmental problem owing to its toxicity for the biodiesel producing sectors where they are generated in bulk as a major by-product. The aim of the present work was to study the feasibility of JSC as a substrate for lipase production under submerged condition. Pseudomonas aeruginosa AAU2 isolated from dumped rotting JSC was found to produce extracellular solvent tolerant lipase (0.432 U ml−1). A 11.4-fold increase in enzyme yield was obtained in an optimized medium containing 20 g JSC/l, 2.5 g potassium nitrate/l and 0.1 v/v% Tween 80 after 60 h of incubation at 30 °C under shake flask conditions (150 rpm). The pH and temperature optima of the partially purified AAU2 lipase were 7.5 and 40 °C, respectively. The AAU2 lipase was found to be a cysteine hydrolase and exhibited preference towards longer carbon chain fatty acid ester substrates over the shorter ones. The enzyme exhibited significant stability in the presence of commercial detergents namely, Ezee® and Wheel®. The AAU2 lipase efficiently catalyzed biodiesel production using Jatropha oil. In addition, the enzyme also hydrolyzed 86.49% of commercially available phorbol ester (phorbol 12-myristate 13-acetate) within 12 h of incubation at 37 °C, suggesting its application in detoxification of phorbol ester from JSC. These results demonstrate viable approach for utilization of JSC biomass and degradation of phorbol esters by P. aeruginosa AAU2.
Journal: Biocatalysis and Agricultural Biotechnology - Volume 2, Issue 3, July 2013, Pages 255–266