کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2075623 | 1544905 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The enzyme precipitated successfully with both polymers. The precipitation method applied to commercial endoglucanase and the fungal extract showed similar patterns with high purification factors. The recovery of the activity in the re-dissolved precipitate from the fungal extract was close to 40% at pH 5.3 using PVS (1% w/w) as precipitating agent and the purification factor was near 9. The purification factor of endoglucanase in the precipitate of the enzymatic extract from SSF with CHS (0.05% w/v) was around 7. These parameters make this precipitation method appropriate to be included in the last stages of a downstream process, with advantages such as simplicity, scalability and ability to concentrate and stabilize the enzyme.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 7, July 2016, Pages 118-126
Journal: Biocatalysis and Agricultural Biotechnology - Volume 7, July 2016, Pages 118-126
نویسندگان
MarÃa Julia Boggione, Ramiro Becher, Beatriz Farruggia,