کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2076956 | 1079473 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Whole genome analysis of non-optimal codon usage in secretory signal sequences of Streptomyces coelicolor Whole genome analysis of non-optimal codon usage in secretory signal sequences of Streptomyces coelicolor](/preview/png/2076956.png)
Non-optimal (rare) codons have been suggested to reduce translation rate and facilitate secretion in Escherichia coli. In this study, the complete genome analysis of non-optimal codon usage in secretory signal sequences and non-secretory sequences of Streptomyces coelicolor was performed. The result showed that there was a higher proportion of non-optimal codons in secretory signal sequences than in non-secretory sequences. The increased tendency was more obvious when tested with the experimental data of secretory proteins from proteomics analysis. Some non-optimal codons for Arg (AGA, CGU and CGA), Ile (AUA) and Lys (AAA) were significantly over presented in the secretary signal sequences. It may reveal that a balanced non-optimal codon usage was necessary for protein secretion and expression in Streptomyces.
Journal: Biosystems - Volume 85, Issue 3, September 2006, Pages 225–230