کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2077347 1079702 2014 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment
ترجمه فارسی عنوان
تجزیه تک سلولی کلاس های مشخصی را در داخل سلول های سلول های پلاناری نشان می دهد
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• Neoblasts, planarian regenerative cells, comprise large distinct cellular classes
• ζ-class neoblasts are lineage-specified precursors that give rise to epidermis
• σ-class neoblasts collectively possess many stem cell-like features

SummaryPlanarians are flatworms capable of regenerating any missing body region. This capacity is mediated by neoblasts, a proliferative cell population that contains pluripotent stem cells. Although population-based studies have revealed many neoblast characteristics, whether functionally distinct classes exist within this population is unclear. Here, we used high-dimensional single-cell transcriptional profiling from over a thousand individual neoblasts to directly compare gene expression fingerprints during homeostasis and regeneration. We identified two prominent neoblast classes that we named ζ (zeta) and σ (sigma). Zeta-neoblasts encompass specified cells that give rise to an abundant postmitotic lineage, including epidermal cells, and are not required for regeneration. By contrast, sigma-neoblasts proliferate in response to injury, possess broad lineage capacity, and can give rise to zeta-neoblasts. These findings indicate that planarian neoblasts comprise two major and functionally distinct cellular compartments.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 15, Issue 3, 4 September 2014, Pages 326–339
نویسندگان
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