کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2077486 1079718 2014 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Contractile Forces Sustain and Polarize Hematopoiesis from Stem and Progenitor Cells
ترجمه فارسی عنوان
نیروهای مخالف حفظ و کنترل هماوتوپیزیس از سلول های شاخ و برگ پیشرو
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• A and B isoforms of myosin-II switch in hematopoietic differentiation
• Polarizable myosin-IIB contributes to asymmetric division
• Niche-sensitive, essential myosin-IIA is increasingly activated in differentiation
• Inhibiting myosin-II enriches for long-term hematopoietic stem and progenitors

SummarySelf-renewal and differentiation of stem cells depend on asymmetric division and polarized motility processes that in other cell types are modulated by nonmuscle myosin-II (MII) forces and matrix mechanics. Here, mass spectrometry-calibrated intracellular flow cytometry of human hematopoiesis reveals MIIB to be a major isoform that is strongly polarized in hematopoietic stem cells and progenitors (HSC/Ps) and thereby downregulated in differentiated cells via asymmetric division. MIIA is constitutive and activated by dephosphorylation during cytokine-triggered differentiation of cells grown on stiff, endosteum-like matrix, but not soft, marrow-like matrix. In vivo, MIIB is required for generation of blood, while MIIA is required for sustained HSC/P engraftment. Reversible inhibition of both isoforms in culture with blebbistatin enriches for long-term hematopoietic multilineage reconstituting cells by 5-fold or more as assessed in vivo. Megakaryocytes also become more polyploid, producing 4-fold more platelets. MII is thus a multifunctional node in polarized division and niche sensing.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 14, Issue 1, 2 January 2014, Pages 81–93
نویسندگان
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