کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2077488 1079718 2014 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Transcriptional and Functional Properties of Mouse Epiblast Stem Cells Resemble the Anterior Primitive Streak
ترجمه فارسی عنوان
خواص ترانسفکشن و کارکردی سلول های بنیادی موش های اپیبلاست شباهت به زنجیره اولیه
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• EpiSCs derived from different embryonic stages show similar molecular characteristics
• EpiSCs are developmentally similar to late-gastrula-stage ectoderm
• Differentiation features anterior primitive streak properties in vitro and in vivo
• Germ-layer predisposition of EpiSCs correlates with gene expression

SummaryMouse epiblast stem cells (EpiSCs) can be derived from a wide range of developmental stages. To characterize and compare EpiSCs with different origins, we derived a series of EpiSC lines from pregastrula stage to late-bud-stage mouse embryos. We found that the transcriptomes of these cells are hierarchically distinct from those of the embryonic stem cells, induced pluripotent stem cells (iPSCs), and epiblast/ectoderm. The EpiSCs display globally similar gene expression profiles irrespective of the original developmental stage of the source tissue. They are developmentally similar to the ectoderm of the late-gastrula-stage embryo and behave like anterior primitive streak cells when differentiated in vitro and in vivo. The EpiSC lines that we derived can also be categorized based on a correlation between gene expression signature and predisposition to differentiate into particular germ-layer derivatives. Our findings therefore highlight distinct identifying characteristics of EpiSCs and provide a foundation for further examination of EpiSC properties and potential.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 14, Issue 1, 2 January 2014, Pages 107–120
نویسندگان
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