کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2077672 1079737 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ESCs Require PRC2 to Direct the Successful Reprogramming of Differentiated Cells toward Pluripotency
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
ESCs Require PRC2 to Direct the Successful Reprogramming of Differentiated Cells toward Pluripotency
چکیده انگلیسی

SummaryEmbryonic stem cells (ESCs) are pluripotent, self-renewing, and have the ability to reprogram differentiated cell types to pluripotency upon cellular fusion. Polycomb-group (PcG) proteins are important for restraining the inappropriate expression of lineage-specifying factors in ESCs. To investigate whether PcG proteins are required for establishing, rather than maintaining, the pluripotent state, we compared the ability of wild-type, PRC1-, and PRC2-depleted ESCs to reprogram human lymphocytes. We show that ESCs lacking either PRC1 or PRC2 are unable to successfully reprogram B cells toward pluripotency. This defect is a direct consequence of the lack of PcG activity because it could be efficiently rescued by reconstituting PRC2 activity in PRC2-deficient ESCs. Surprisingly, the failure of PRC2-deficient ESCs to reprogram somatic cells is functionally dominant, demonstrating a critical requirement for PcG proteins in the chromatin-remodeling events required for the direct conversion of differentiated cells toward pluripotency.

Graphical AbstractFigure optionsDownload high-quality image (166 K)Download as PowerPoint slideHighlights
► Mouse pluripotent cells reprogram human B cells in heterokaryons
► ESCs lacking PRC1 or PRC2 cannot successfully reprogram
► Failure of PRC1/2-deficient ESCs to reprogram is functionally dominant
► PRC1/2 play a critical role in the chromatin reorganization required for reprogramming

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 6, Issue 6, 4 June 2010, Pages 547–556
نویسندگان
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