کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2077911 | 1079756 | 2010 | 14 صفحه PDF | دانلود رایگان |

SummaryBone marrow endothelial cells (ECs) are essential for reconstitution of hematopoiesis, but their role in self-renewal of long-term hematopoietic stem cells (LT-HSCs) is unknown. We have developed angiogenic models to demonstrate that EC-derived angiocrine growth factors support in vitro self-renewal and in vivo repopulation of authentic LT-HSCs. In serum/cytokine-free cocultures, ECs, through direct cellular contact, stimulated incremental expansion of repopulating CD34−Flt3−cKit+Lineage−Sca1+ LT-HSCs, which retained their self-renewal ability, as determined by single-cell and serial transplantation assays. Angiocrine expression of Notch ligands by ECs promoted proliferation and prevented exhaustion of LT-HSCs derived from wild-type, but not Notch1/Notch2-deficient, mice. In transgenic notch-reporter (TNR.Gfp) mice, regenerating TNR.Gfp+ LT-HSCs were detected in cellular contact with sinusoidal ECs. Interference with angiocrine, but not perfusion, function of SECs impaired repopulation of TNR.Gfp+ LT-HSCs. ECs establish an instructive vascular niche for clinical-scale expansion of LT-HSCs and a cellular platform to identify stem cell-active trophogens.
Graphical AbstractFigure optionsDownload high-quality image (312 K)Download as PowerPoint slideHighlights
► Coculture with endothelial cells promotes HSC expansion and self-renewal
► Endothelial cell effect on HSCs mediated by Notch signaling
► In vivo, sinusoidal endothelial cells (SECs) express Notch ligands
► Blocking vessel remodeling impairs hematopoietic recovery after irradiation
Journal: - Volume 6, Issue 3, 5 March 2010, Pages 251–264