Functional Integration of Dopaminergic Neurons Directly Converted from Mouse Fibroblasts

SummaryRecent advances in somatic cell reprogramming have highlighted the plasticity of the somatic epigenome, particularly through demonstrations of direct lineage reprogramming of one somatic cell type to another by defined factors. However, it is not clear to what extent this type of reprogramming is able to generate fully functional differentiated cells. In addition, the activity of the reprogrammed cells in cell transplantation assays, such as those envisaged for cell-based therapy of Parkinson's disease (PD), remains to be determined. Here we show that ectopic expression of defined transcription factors in mouse tail tip fibroblasts is sufficient to induce Pitx3+ neurons that closely resemble midbrain dopaminergic (DA) neurons. In addition, transplantation of these induced DA (iDA) neurons alleviates symptoms in a mouse model of PD. Thus, iDA neurons generated from abundant somatic fibroblasts by direct lineage reprogramming hold promise for modeling neurodegenerative disease and for cell-based therapies of PD.

► Induction of dopaminergic (DA) neurons from mouse tail tip fibroblasts
► Highest efficiency with a combination of six transcription factors plus FGF8 and Shh
► Induced neurons show hallmarks of mature DA neurons
► Functional integration and phenotypic rescue in a mouse model of Parkinson's disease