کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2078253 1079779 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sequential Expression of Pluripotency Markers during Direct Reprogramming of Mouse Somatic Cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Sequential Expression of Pluripotency Markers during Direct Reprogramming of Mouse Somatic Cells
چکیده انگلیسی

SummaryPluripotency can be induced in differentiated murine and human cells by retroviral transduction of Oct4, Sox2, Klf4, and c-Myc. We have devised a reprogramming strategy in which these four transcription factors are expressed from doxycycline (dox)-inducible lentiviral vectors. Using these inducible constructs, we derived induced pluripotent stem (iPS) cells from mouse embryonic fibroblasts (MEFs) and found that transgene silencing is a prerequisite for normal cell differentiation. We have analyzed the timing of known pluripotency marker activation during mouse iPS cell derivation and observed that alkaline phosphatase (AP) was activated first, followed by stage-specific embryonic antigen 1 (SSEA1). Expression of Nanog and the endogenous Oct4 gene, marking fully reprogrammed cells, was only observed late in the process. Importantly, the virally transduced cDNAs needed to be expressed for at least 12 days in order to generate iPS cells. Our results are a step toward understanding some of the molecular events governing epigenetic reprogramming.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 2, Issue 2, 7 February 2008, Pages 151–159
نویسندگان
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