کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2078818 | 1545045 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biotechnological Analysis of Caffeic Acid Metabolites Possessing Potent Antinephritic Activity
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
During an investigation of plant cell cultures, which may be useful for the treatment of renal disorders, we established a well-growing E-4 callus culture of Eritrichium sericeum that produced high amounts of caffeic acid metabolites (CAM), (â)-rabdosiin (1.8% dry wt), and rosmarinic acid (RA) (4.6% dry wt). Elicitation of the calli induced an increase in (â)-rabdosiin production by as much as 4.1% dry wt. Oral administration of E-4 callus biomass to rats with induced Masugi-nephritis caused an increased diuresis, lowered creatinine excretion and proteinuria levels, compared with Masugi-nephritis untreated rats. Although all the Masugi-nephritis untreated rats began to suffer, near a quarter of the E-4-treated rats remained in good health. This result indicates that the E-4 culture has a potential to alleviate symptoms associated with nephritis. A mechanism by which production of CAM could be activated in the calli was studied using a high polyphenol-producing cell cultures transformed with rolC gene. We established that the increase of CAM production in rolC-transgenic E. sericeum calli positively correlated with high expression of the CYP98A3 gene, a key gene for RA biosynthesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 24, Issue 12, December 2008, Pages 2140-2141
Journal: Chinese Journal of Biotechnology - Volume 24, Issue 12, December 2008, Pages 2140-2141
نویسندگان
Yulia V. Inyushkina, Konstantin V. Kiselev, Victor P. Bulgakov,