Progress of Antisense Technology Applied in Metabolic Regulation of Bacteria

With the rapid development of genetic engineering and metabolic regulation, antisense technology displays its fascination to the world as a mild regulation genetic tool. Compared with other loss-of-function research methods (e.g. gene knockout), antisense methods have advantages such as low cost, short period, and easy operation. Hence, they have been increasingly used in bacterial metabolic regulation as a powerful genetic tool. This review briefly summarized the latest progresses and challenges in antisense methods that are recently used in metabolic engineering of bacteria and compared the advantages and disadvantages of these approaches.