کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2078872 | 1545057 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of Binding Spectrum in DMIs Fungicide Screening
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
In order to establish a fast and accurate method for novel DMIs fungicide screening, lanosterol 14α-demethylase of Magnaporthe grisea expressed in E. coli was used as target enzyme and the DMI fungicides diniconazole, tebuconazole, triadimenol and triadimefon were used as representative fungicides, the effects of enzyme activity, enzyme purity and concentration on the binding spectra were investigated. The results showed that active enzyme, elimination of interference of other P-450s and proper enzyme concentration were necessary for obtaining accurate binding spectra. The Kd values of diniconazole, tebuconazole, triadimenol and triadimefon were 0.143 μmol/L, 0.24 μmol/L, 0.257 μmol/L and 0.307 μmol/L respectively, which significantly correlated to their 120h-EC50 values on the growth of M.grisea. The results indicated that the binding spectra of fungicide and lanosterol 14α-demethylase can serve as a reliable and fast method for novel fungicide screening.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 23, Issue 6, November 2007, Pages 1129-1134
Journal: Chinese Journal of Biotechnology - Volume 23, Issue 6, November 2007, Pages 1129-1134
نویسندگان
XIAO Min, YANG Jiao-Yan, XIAO Wen-Jing, YANG Shao,