کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2078887 | 1545055 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of Goose Interleukin 2 in Escherichia coli and Isolation of Its Soluble Monomer
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression of Goose Interleukin 2 in Escherichia coli and Isolation of Its Soluble Monomer Expression of Goose Interleukin 2 in Escherichia coli and Isolation of Its Soluble Monomer](/preview/png/2078887.png)
چکیده انگلیسی
Recombinant expression plasmid of pET-28a(+)-goIL-2 was constructed by inserting goose IL-2 gene without signal peptide sequence into a prokaryotic expression vector pET-28a(+), and transformed into E. coli BL21(DE3) cells for expression. After IPTG induction, an expected protein band with molecular weight of 15.0 kD was observed on SDS-PAGE gel, recognized by monoclonal antibody against goose IL-2 in Western blotting analysis. In the pET-28a(+) expression system, the recombinant goose IL-2(rgoIL-2) was mainly found in inclusion bodies with a portion of soluble protein. The monomer and multimers of soluble goose interleukin 2 protein were observed in native electrophoresis. The rgoIL-2 protein was purified by Ni-NTA column under native condition. The rgoIL-2 soluble protein monomer was isolated by a quick protein isolation and purification system of ÃKTA FPLC and identified by native PAGE. Bioactivity analysis showed that the rgoIL-2 monomer stimulated the proliferation of goose lymphocytes in vitro. It provides a basis for studying the biological function of goose IL-2 as well as its clinical application.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 24, Issue 2, February 2008, Pages 183-187
Journal: Chinese Journal of Biotechnology - Volume 24, Issue 2, February 2008, Pages 183-187
نویسندگان
Jing Qi, Jigang Chen, Jinyong Wang, Jie Fang, Jiajun Wu, Jiyong Zhou,