کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2078944 | 1545063 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and expression of the genes encoding glycerol dehydratase reactivase and identification of its biological activity
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cloning and expression of the genes encoding glycerol dehydratase reactivase and identification of its biological activity Cloning and expression of the genes encoding glycerol dehydratase reactivase and identification of its biological activity](/preview/png/2078944.png)
چکیده انگلیسی
The gdrA and gdrB genes encoding glycerol dehydratase reactivase were amplified using the genomic DNA of Klebsiella pneumoniae as the template. The gdrA and gdrB genes were inserted into pMD-18T to yield the recombinant cloning vector pMD-gdrAB. After the DNA sequence was determined, the gdrAB gene was subcloned into expression vector pET-28a(+) to yield the recombinant expression vector pET-28gdrAB. Under screening pressure by ampicillin and kanamycin simultaneously, the activity of glycerol dehydratase reactivase was characterized by the coexpression of pET-32gldABC, which carried the gldABC gene encoding glycerol dehydratase, and pET-28gdrAB in E. coli BL21 (DE3).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 6, November 2006, Pages 950-955
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 6, November 2006, Pages 950-955
نویسندگان
LI Wen-Jun, FANG Bai-Shan, HONG Yan, WANG Xiao-Xia, LIN Jin-Xia, LIU Gui-Lan,