کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2078958 | 1545059 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid Improvement in Lipase Production of Penicillium expansum by Genome Shuffling
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
In the present study, the genome shuffling was used to improve the lipase production of Penicillium expansum (P. expansum). A lipase-producing mutant strain-P. expansum FS8486 and a wild type of Aspergillus tamarii FS-132 isolated from soil of a volcano in Xinjiang were used as the parental strains. After two rounds of genome shuffling, several elite daughter strains were screened. The lipase activity in one of the daughter strains was increased 317% over the starting strain FS8486. Comparisons of the morphology, random amplification of polymorphic DNA polymorphism, and fatty acid compositions between the daughter and the parental strains suggested that the filial generation were generated by genome shuffling. In this study, the genome shuffling was used successfully in eukaryotic microorganism and the production of the desired metabolite was increased within the short period, the study will be useful to spread the genome shuffling in eukaryotic microbial breeding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 23, Issue 4, July 2007, Pages 672-676
Journal: Chinese Journal of Biotechnology - Volume 23, Issue 4, July 2007, Pages 672-676
نویسندگان
LIN Jun, SHI Bi-Hong, SHI Qiao-Qin, HE Yun-Xia, WANG Ming-Zi,