کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2078979 | 1545061 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The Targeting Study of Human Serum Albumin Gene
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
To generate transgenic porcine which expresses human serum albumin (HAS), the HSA gene targeting vector was constructed with HSA cDNA as the gene of interest and partial porcine serum albumin (PSA) gene as homologous arms which were 7.2 kb 5â² regulation sequence and 2.8 kb genomic sequence from the first intron to the fourth intron, respectively. The resistant gene neo was inserted into intron 1 and tk was ligated to the 3â² end of the construct. Linearized targeting construct DNA was introduced into the fibroblast cells obtained from porcine fetus by electroporation. The positive-negative selection was performed and survival clones were screened by PCR and Southern blot. Three colonies with correct homologous recombination were obtained. Our results set a good basis for the establishment of transgenic porcine by gene target and nuclear transfer methods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 23, Issue 2, March 2007, Pages 222-227
Journal: Chinese Journal of Biotechnology - Volume 23, Issue 2, March 2007, Pages 222-227
نویسندگان
SUN Shi-Hui, ZHOU Yan-Rong, CHEN Hong-Xing, LIN Yan-Li, HUANG Pei-Tang, DENG Ji-Xian,