Rapid Detection of Pseudomonas aernginosa by the Fluorescence Quantitative TaqMan PCR Assay Targetting ETA Gene

Pseudomonas aernginosa (PA) is one of the most universal pathogens in clinical diagnosis, and conventional detection assay has several disadvantages. In this study, a pair of specific primers and a TaqMan fluorescent probe were designed in the conservative region of ETA gene by the method of bioinformatics analysis, and the detection method for PA was successfully developed. Different gradient concentrations of PA DNA and various pathogen DNA were amplified by FQ-PCR to confirm the specificity and sensitivity of the developed method. The results showed that the developed detection assay is more sensible and specific by comparison to the conventional FQ-PCR method; it is valuable for research and application prospects.