Preparing Engineered Tissues in vitro by Macroporous Microcarriers

Macroporous microcarriers (Cytopore) were used to construct microfabricated tissues. Perfusion approach was used to reassemble macrotissue based on their aggregative property. Microfabricated tissues reached the maximum cell density with 16.4× 107 cells/cm3 after 20 days. They were uniform with high cell viability and massive matrix as a whole after 32 days. The aggregation of the microfabricated tissues was visible especially in the later period of the culture, and a centimeter-sized (12 mm× 6 mm) macrotissue with uniform cell and matrix distribution was obtained through perfusion reassembly. Constructing engineered tissues using macroporous microcarriers is effective and promising.