کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2079032 | 1545065 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of Phosphate on Cell Growth and Polysaccharide Production by Suspension Cultures of Protocorm-like Bodies of Dendrobium huoshanense
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effect of Phosphate on Cell Growth and Polysaccharide Production by Suspension Cultures of Protocorm-like Bodies of Dendrobium huoshanense Effect of Phosphate on Cell Growth and Polysaccharide Production by Suspension Cultures of Protocorm-like Bodies of Dendrobium huoshanense](/preview/png/2079032.png)
چکیده انگلیسی
The effect of inorganic phosphate on the cell growth and accumulation of polysaccharides together with nutrient utilization was investigated in suspension cultures of protocorm-like bodies of Dendrobium huoshanense. Thirty-day-old cells were transferred into a liquid medium with the inoculum density of 100 g/L cells. The results indicated that the optimal concentration of phosphate in the medium for the growth of protocorm-like bodies of Dendrobium huoshanense was 2.5 mmol/L and the biomass was 496.5 g/L (fresh weight). The phosphate was a limiting factor for the cell growth and there was a relationship between the levels of intracellular phosphate and the cell growth. A total of 2.5 mmol/L of medium phosphate was beneficial to the absorption of carbon and nitrogen source. A total of 0.312 mmol/L of medium phosphate was better for the accumulation of polysaccharides and the production of polysaccharides was 2.22 g/L on day 36.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 4, July 2006, Pages 613-617
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 4, July 2006, Pages 613-617
نویسندگان
JIANG Shao-Tong, WEI Ming, LUO Jian-Ping,