کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2079050 | 1545067 | 2006 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-level Expression of Phenylalanine Ammonia-lyase in Lactococcus lactis via Synthesized Sequence Based on Bias Codons
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
To construct a safer and more efficient gene engineering Lactococcus lactis for expressing phenylalanine ammonia lyase (PAL) which will benefit PKU therapy. A PAL cDNA of Parsly and a synthesized PAL sequence based on Lactococcus lactis bias codons (palart) were recombined into two Lactococcus lactis NICE systems. The activities of the expressed PALs were detected, and the effect of Lactococcus lactis bias codons on the expression of exterior protein was analyzed. The results showed that the expression level of PAL was increased by using bias codons in the two Lactococcus lactis NICE systems, through which several safer and more efficient strains of the gene engineering Lactococcus lactis were obtained.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 2, March 2006, Pages 187-190
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 2, March 2006, Pages 187-190
نویسندگان
CHEN Xing, GAO Bin, JIA Xing-Yuan, SU Chang, LÃ Yue-Ping, WANG Zhan-Yong, FAN Xin-Ping, XIAO Bai, LIU Jing-Zhong,