کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2079064 | 1545066 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of the Relationship Between Nm23-H1 Gene and Human Chronic Myeloblastic Leukemia Using SiRNA
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To investigate the relationship between nm23-H1 gene and human chronic myeloblastic leukemia, siRNAs were designed to target the nm23-H1 gene. According to the principles of designing siRNA, three siRNAs were selected and transfected into K562 cells by lipofectamine 2 000. The expression levels of nm23-H1 mRNA were detected by reverse transcriptase-polymerase chain reaction after transfection for 24 h. The expression levels of nm23-H1 protein were assayed by the immunocytochemical method after transfection for 48 h. After transfection for 24, 48 and 72 h, cell proliferation was determined by the MTT method. Among the three siRNAs, siNM526 effectively inhibited the expression of nm23-H1 at mRNA and protein levels. The growth of K562 cells was suppressed after the transfection of siNM526. These results suggest that the low expression level of nm23-H1 in K562 cells inhibited cell proliferation, that is, reduced the degree of malignancy. Therefore, the nm23-H1 gene might be a potential target for leukemia treatment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 3, May 2006, Pages 403-407
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 3, May 2006, Pages 403-407
نویسندگان
CHEN Yu-Xia, ZHANG Mei-Ying, XIONG Sheng, QIAN Chui-Wen, WANG Yi-Fei,