کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2079523 1545081 2014 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Overexpression of GmDREB1 improves salt tolerance in transgenic wheat and leaf protein response to high salinity
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Overexpression of GmDREB1 improves salt tolerance in transgenic wheat and leaf protein response to high salinity
چکیده انگلیسی

The transcription factor dehydration-responsive element binding protein (DREB) is able to improve tolerance to abiotic stress in plants by regulating the expression of downstream genes involved in environmental stress resistance. The objectives of this study were to evaluate the salt tolerance of GmDREB1 transgenic wheat (Triticum aestivum L.) and to evaluate its physiological and protein responses to salt stress. Compared with the wild type, the transgenic lines overexpressing GmDREB1 showed longer coleoptiles and radicles and a greater radicle number at the germination stage, as well as greater root length, fresh weight, and tiller number per plant at the seedling stage. The yield-related traits of transgenic lines were also improved compared with the wild type, indicating enhanced salt tolerance in transgenic lines overexpressing GmDREB1. Proteomics analysis revealed that osmotic- and oxidative-stress-related proteins were up-regulated in transgenic wheat leaves under salt stress conditions. Transgenic wheat had higher levels of proline and betaine and lower levels of malondialdehyde and relative electrolyte leakage than the wild type. These results suggest that GmDREB1 regulates the expression of osmotic- and oxidative-stress-related proteins that reduce the occurrence of cell injury caused by high salinity, thus improving the salt tolerance of transgenic wheat.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Crop Journal - Volume 2, Issues 2–3, April–June 2014, Pages 120–131
نویسندگان
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