Optical imaging of bacterial infection models

Over the last 13 years, the field of optical imaging has expanded from in vitro fluorescence microscopy of cells to in vivo whole body imaging of living animals. Recent advances in optical imaging of bacterial infection have been propelled by the invention of genetic methods that produce fluorescent and bioluminescent bacteria, and also the discovery of synthetic fluorescent probes that selectively target bacterial cell surfaces. Optical imaging is an effective method for conducting longitudinal studies of bacterial infection in small animals such as nude mice. It can be used to address questions in medical microbiology concerning migration and colonization and it is an attractive method for determining the efficacy of antibiotic therapies.

Section editor:Trevor Trust – AstraZeneca R&D Boston, 35 Gatehouse Drive, Waltham, MA 02451, USA