Two-hybrid and its recent adaptations

Interactions between proteins play a pivotal role in virtually all cellular processes, and many of these interactions represent interesting targets for drug development. Among the wide array of interactor-hunting technologies that has emerged, genetic two-hybrid methods account for a large amount of the currently available interaction data and is being successfully applied in interactome-scale mapping projects. Reverse two-hybrid approaches have been developed that allow selected interactions to be assayed for disrupting compounds.

Section editors:Anne-Claude Gavin – EMBL, Heidelberg, GermanyAlbert Heck – Utrecht University, Utrecht, Germany