Protein co-membership and biochemical affinity purifications

Cellular functions are almost always the result of the coordinated action of several proteins, interacting in molecular assemblies or pathways. Significant progress has been made in devising suitable tools for global analysis of protein–protein interactions. Among them, a suite of powerful technologies is now available enabling purification of protein complexes under native conditions and, in conjunction with protein mass spectrometry, identification of their constituents.

Section editors:Anne-Claude Gavin – EMBL, Heidelberg, GermanyAlbert Heck – Utrecht Unvisersity, Utrecht, The Netherlands