Difference gel electrophoresis DIGE

Proteomics offers powerful technologies to assist in the discovery of targets for novel therapeutic agents, by allowing the investigation of changes in protein state between control and diseased tissue and biofluids. Difference gel electrophoresis coupled with mass spectrometry (DIGE/MS) is a technology used within proteomics that has demonstrated technical robustness and associated statistical confidence to enable successful identification of therapeutic targets.

Section editors:Anne-Claude Gavin – EMBL, Heidelberg, GermanyAlbert Heck – Utrecht University, Utrecht, The Netherlands