The effect of GlycoPEGylation on the physical stability of human rFVIIa with increasing calcium chloride concentration

GlycoPEGylation postpones the calcium induced thermal destabilisation of recombinant human factor VIIa. Increasing the CaCl2 concentration from 10 mM to 35 mM resulted in a decrease in the apparent unfolding temperature, Tm, of rFVIIa, whereas the concentration of CaCl2 has to be raised to 100 mM in order to see the same effect on the GlycoPEGylated rFVIIa compounds.