کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2084568 1545393 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-specific translocation of peptide-displaying bacteriophage particles across the gastrointestinal barrier
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Non-specific translocation of peptide-displaying bacteriophage particles across the gastrointestinal barrier
چکیده انگلیسی

Phage display technology could provide a rapid means for the discovery of novel peptides and proteins from genetically engineered variants which may act as specific vehicles for drug delivery particularly through the intestinal barrier. In this work, we utilized in vivo phage display in order to study the sequences which may be responsible for transmucosal transport. We hypothesized that the introduction of a library of peptide displaying phages into the intestine may lead to the identification of sequences that could induce transport. A biopanning protocol was performed by applying a 7-mer random amino acid phage library to mice by gavage and then assessing their absorption via phage recovery from the spleen and blood. Following the isolation of 77 different phages, the sequences of the displayed peptides were identified. Statistical treatment of the obtained sequences did not support the notion that the GI translocation depends on the presence of any particular peptide sequence fused on the pIII coat proteins of the M13 phages. There are, however, some residue types underrepresented which could be due to specific GI selection mechanisms and/or their effects on the amplification rate for phages bearing those residues.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 70, Issue 2, October 2008, Pages 577–581
نویسندگان
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