کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2085292 1545358 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Uricase from Bacillus fastidious loaded in alkaline enzymosomes: Enhanced biochemical and pharmacological characteristics in hypouricemic rats
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Uricase from Bacillus fastidious loaded in alkaline enzymosomes: Enhanced biochemical and pharmacological characteristics in hypouricemic rats
چکیده انگلیسی

The aim of this study was to assess the potential of a novel alkaline enzymosome to deliver uricase from Bacillus fastidious (UBF) and enhance its biochemical and pharmacological characteristics. The in vitro catalytic activity of the UBF loaded in the novel alkaline enzymosomes (ESUBFs) was almost 3.8 times that of free UBF at the optimum pH or 1.5 times that of free UBF at the physiological pH. Following intravenous (i.v.) administration (2000 mU/kg) in rats, ESUBFs provided significantly higher (22.5-fold) area under the plasma concentration (AUC) and longer (8.2-fold) circulation half-life (t1/2) compared with free UBF, respectively. Further, it took only 4.5 h (or 1.1 h) for ESUBFs to lower the plasma uric acid concentration from a high level to the normal level of rat (or human beings), compared with 7.6 h (or 5.4 h) for free UBF. Our results showed that ESUBFs could efficiently deliver UBF and favorably modify its biochemical and pharmacological characteristics by increasing the AUC, t1/2, and catalytic activity. Therefore, ESUBFs might be a preferred alternative to cure hyperuricemia and gout.

Alkaline enzymosomes containing uricase from Bacillus fastidious: improved biochemical properties in vitro and pharmacological characteristics in vivo.Figure optionsDownload high-quality image (100 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 82, Issue 1, September 2012, Pages 43–48
نویسندگان
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