کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2085312 1545358 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcutaneous DNA immunization following waxing-based hair depilation elicits both humoral and cellular immune responses
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Transcutaneous DNA immunization following waxing-based hair depilation elicits both humoral and cellular immune responses
چکیده انگلیسی

Previously, we showed that transcutaneous (TC) DNA immunization by applying plasmid DNA onto a mouse skin area wherein the hair follicles were induced into growth stage by plucking the hair using warm waxing induced strong and functional antigen-specific antibody responses. In the present study, using plasmids that encode β-galactosidase gene or ovalbumin (OVA) gene, we showed that this mode of TC DNA immunization not only induced specific antibody responses, but also induced antigen-specific cytotoxic T lymphocyte responses. In fact, TC DNA immunization using a plasmid that encodes OVA gene prevented the growth of OVA-expressing B16-OVA tumor cells in the immunized mice. Moreover, we provided additional evidence supporting that hair follicles are essential for this mode of TC DNA immunization.

DNA immunization by applying plasmid DNA topically onto a mouse skin area wherein the hair had been plucked by warm waxing induced specific antibody and cellular immune responses.Figure optionsDownload high-quality image (88 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 82, Issue 1, September 2012, Pages 212–217
نویسندگان
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