کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2085522 1545378 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cellular uptake mechanisms and intracellular distributions of polysorbate 80-modified poly (d,l-lactide-co-glycolide) nanospheres for gene delivery
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Cellular uptake mechanisms and intracellular distributions of polysorbate 80-modified poly (d,l-lactide-co-glycolide) nanospheres for gene delivery
چکیده انگلیسی

We previously developed chitosan (CS)-modified poly (d,l-lactide-co-glycolide) (PLGA) nanospheres (NS) by an emulsion solvent diffusion method as a gene delivery system. In this study, PLGA NS were modified using polysorbate 80 (P80) to improve their cellular uptake. We investigated the cellular uptake, intracellular distribution, and transfection efficiency of P80-modified PLGA NS (P80-PLGA NS) for a plasmid DNA delivery system in A549 cells. The cellular uptake and transfection efficiency of P80-PLGA NS were greater than CS-modified PLGA NS (CS-PLGA NS). The uptake of unmodified NS and CS-PLGA NS was mediated, predominantly, by clathrin-mediated endocytosis. In contrast, a specific endocytic pathway could not be determined for the cellular uptake of P80-PLGA NS. The intracellular distributions of PLGA NS depended on their surface properties. P80-PLGA NS were not cytotoxic for A549 cells. Thus, P80-PLGA NS could be used as an effective gene delivery system; the surface properties of PLGA NS are key parameters for optimal intracellular uptake and distribution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 75, Issue 2, June 2010, Pages 218–224
نویسندگان
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