کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2086141 1545505 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring
چکیده انگلیسی

A functional gene containing an Open Reading Frame (ORF) encoding a β-1, 4-endoxylanase glycosyl hydrolase family 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The gene consisted of 642 nucleotides, encoded for 213 amino acids. The amino acid sequence analysis using BLAST showed that the gene has high homology (93%) with xylanase gene from Bacillus subtilis. The gene showed its function when it was subcloned into an expression vector and overexpressed in E. coli. The crude extract of the recombinant enzyme had activity for 170 U/ml at 50 °C. The result of this work showed that metagenomic approach was a powerful short cut method to obtain recombinant biocatalyst that was useful for industrial application.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: HAYATI Journal of Biosciences - Volume 14, Issue 2, June 2007, Pages 54–58
نویسندگان
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