کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2087688 1080682 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HPLC analysis and antimicrobial, antimycobacterial and antiviral activities of Tabernaemontana catharinensis A. DC
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
HPLC analysis and antimicrobial, antimycobacterial and antiviral activities of Tabernaemontana catharinensis A. DC
چکیده انگلیسی

Antimicrobial, antimycobacterial and antiviral activities of crude extracts, fractions and subfractions of Tabernaemontana catharinensis were evaluated. Best antimicrobial results occurred with the dichloromethane (DCM) and butanolic (NB) fractions against Staphylococcus aureus, Micrococcus sp., Enterococcus faecalis and Bacillus subtilis (minimal inhibitory concentration, MIC = 31.25–1000 μg/mL). Considering the Gram-negative bacteria, only NB fraction was effective against Proteus mirabilis and Aeromonas sp. (MIC = 62.5 μg/mL and 250 μg/mL, respectively). In addition, the fungi Candida albicans, Candida glabrata, Cryptococcus neoformans, Saccharomyces cerevisiae, Aspergillus flavus and Aspergillus fumigatus were particularly vulnerable for DCM fraction (MIC = 31.25–1000 μg/mL). The fractions and subfractions were effective against Mycobacterium smegmatis (MIC = 19.53–156.25 μg/mL). DCM (selectivity index – SI = 77.92), ethyl acetate (EA) (SI = 40.27) and NB (SI = 28.97) fractions from the leaves exhibited a potential antiviral activity toward Herpes Simplex Virus type 1 whereas DCM2 subfraction from leaves (SI = 12.28) and alkaloidal fraction (AF) (10.71) maintained this good activity. Steroids, terpenoids and phenolics compounds were identified by high-performance liquid chromatography with diode array detection (HPLC/DAD) and may be partially responsible for the antimicrobial and antiherpes activities observed. The results obtained in this study showed that T. catharinensis has antimicrobial and anti-herpetic activities and that these properties are reported for the first time for this species.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Applied Biomedicine - Volume 13, Issue 1, February 2015, Pages 7–18
نویسندگان
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